Uptake of α-ketoglutarate by the citrate transporter CitP drives
نویسندگان
چکیده
4 Agata M. Pudlik and Juke S. Lolkema 5 6 1 Top Institute Food and Nutrition, Wageningen, The Netherlands. 2 Molecular Microbiology, 7 Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 8 Groningen, The Netherlands. 3 The Kluyver Centre for Genomics of Industrial Fermentations 9 / NCSB, Delft, The Netherlands. 10 11 * correspondence 12 J.S. Lolkema, Center for Life Sciences, University of Groningen, Nijenborgh 7, 9747AG 13 Groningen, The Netherlands 14 tel. +31503632155; fax. +31503632154; email. [email protected] 15 16 17 running title: Uptake and transamination of α-ketoglutarate 18 19 Copyright © 2012, American Society for Microbiology. All Rights Reserved. Appl. Environ. Microbiol. doi:10.1128/AEM.02254-12 AEM Accepts, published online ahead of print on 30 November 2012
منابع مشابه
Uptake of α-ketoglutarate by citrate transporter CitP drives transamination in Lactococcus lactis.
Transamination is the first step in the conversion of amino acids into aroma compounds by lactic acid bacteria (LAB) used in food fermentations. The process is limited by the availability of α-ketoglutarate, which is the best α-keto donor for transaminases in LAB. Here, uptake of α-ketoglutarate by the citrate transporter CitP is reported. Cells of Lactococcus lactis IL1403 expressing CitP show...
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Citrate metabolism in Leuconostoc mesenteroides subspecies mesenteroides is associated with the generation of a proton motive force by a secondary mechanism (C. Marty-Teysset, C. Posthuma, J. S. Lolkema, P. Schmitt, C. Divies, and W. N. Konings, J. Bacteriol. 178:2178-2185, 1996). The pathway consists of four steps: (i) uptake of citrate, (ii) splitting of citrate into acetate and oxaloacetate,...
متن کاملThe Citrate Metabolic Pathway in Leuconostoc mesenteroides: Expression, Amino Acid Synthesis, and a-Ketocarboxylate Transport
Citrate metabolism in Leuconostoc mesenteroides subspecies mesenteroides is associated with the generation of a proton motive force by a secondary mechanism (C. Marty-Teysset, C. Posthuma, J. S. Lolkema, P. Schmitt, C. Divies, and W. N. Konings, J. Bacteriol. 178:2178–2185, 1996). The pathway consists of four steps: (i) uptake of citrate, (ii) splitting of citrate into acetate and oxaloacetate,...
متن کاملSubstrate specificity of the citrate transporter CitP of Lactococcus lactis.
The citrate transporter CitP of lactic acid bacteria catalyzes electrogenic precursor-product exchange of citrate versus L-lactate during citrate-glucose cometabolism. In the absence of sugar, L-lactate is replaced by the metabolic intermediates/end products pyruvate, α-acetolactate, and acetate. In this study, the binding and translocation properties of CitP were analyzed systematically for a ...
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Citrate metabolism in resting cells of Lactococcus lactis IL1403(pFL3) results in the formation of two end products from the intermediate pyruvate, acetoin and acetate (A. M. Pudlik and J. S. Lolkema, J. Bacteriol. 193:706-714, 2011). Pyruvate is formed from citrate following uptake by the transporter CitP through the subsequent actions of citrate lyase and oxaloacetate decarboxylase. The prese...
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